Dextran Sodium Sulfate Induced Murine Colitis

C57BL/6 mice were used for DSS colitis. Acute colitis was induced by feeding mice with 3% (wt/vol) dextran sodium sulfate (DSS) (molecular weight 40 kDa; ICN Biochemicals, Aurora, OH) dissolved in drinking water, which was fed ad libitum for seven days (10 (link)). For chronic DSS-induced colitis, mice were treated with 4 cycles of DSS (3%) for 7 days/cycle and 10 days of normal drinking water in between each cycle (10 (link)). Control C57BL/6 mice received the same drinking water without DSS (n=8 mice in each group).

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Alex P., Zachos N.C., Nguyen T., Gonzales L., Chen T.E., Conklin L.S., Centola M, & Li X. (2009). Distinct Cytokine Patterns Identified from Multiplex Profiles of Murine DSS and TNBS-Induced Colitis. Inflammatory bowel diseases, 15(3), 341-352.

Publication 2008

C57bl mice Colitis Dextran sodium sulfate Mice

Corresponding Organization : Johns Hopkins University

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Protocol cited in 23 other protocols

Variable analysis

independent variables

Induction of acute colitis by feeding mice with 3% (wt/vol) dextran sodium sulfate (DSS) in drinking water for seven days
Induction of chronic DSS-induced colitis by treating mice with 4 cycles of 3% DSS for 7 days/cycle and 10 days of normal drinking water in between each cycle

dependent variables

Outcome of acute colitis
Outcome of chronic DSS-induced colitis

control variables

C57BL/6 mice received the same drinking water without DSS

controls

Negative control: C57BL/6 mice received the same drinking water without DSS

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