Bacterial Identification from Blood Cultures

Blood culture was performed using Brain Heart Infusion broth (BHI) (Oxoid Ltd) in a ratio of blood to BHI of 1:10 as previously described [3 (link)]. Subsequent sub-culture was done on day 1, 3 and 7 on 5% sheep blood agar, chocolate agar and MacConkey agar (Oxoid, UK). Identification of bacteria was performed using conventional physiological and biochemical methods [13 (link), 14 ]. Repeat blood culture was ordered in all cases where Coagulase negative staphylococcus (CNS) was isolated. Re-isolation of CNS was considered significant blood culture result. Antimicrobial susceptibility of isolates was determined using disk diffusion method according to the Clinical Laboratory standard Institute (CLSI) [15 ].

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Chacha F., Mirambo M.M., Mushi M.F., Kayange N., Zuechner A., Kidenya B.R, & Mshana S.E. (2014). Utility of qualitative C- reactive protein assay and white blood cells counts in the diagnosis of neonatal septicaemia at Bugando Medical Centre, Tanzania. BMC Pediatrics, 14, 248.

Publication 2014

sheep blood agar chocolate agar MacConkey agar

Agar Antimicrobial Bacteria Blood Blood culture Brain Chocolate Clinical laboratory method Coagulase Diffusion Heart In blood Isolation Physiological Sheep Staphylococcus Susceptibility

Corresponding Organization :

Other organizations : Bugando Medical Centre, Catholic University of Health and Allied Sciences

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Variable analysis

independent variables

Blood culture using Brain Heart Infusion broth (BHI) in a ratio of blood to BHI of 1:10

dependent variables

Identification of bacteria using conventional physiological and biochemical methods
Antimicrobial susceptibility of isolates using disk diffusion method according to the Clinical Laboratory standard Institute (CLSI)

control variables

Repeat blood culture was ordered in all cases where Coagulase negative staphylococcus (CNS) was isolated
Re-isolation of CNS was considered significant blood culture result

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