The fluorescent compounds tetramethylrosamine chloride (TMR-Cl), BODIPY (BD)-verapamil, 3-propionyl ethylenediamine hydrochloride (BD-EDA), BD-prazosin, BD-vinblastine, tetramethylrhodamine ethyl ester perchlorate (TMRE), 3,3'-diethyloxacarbocyanine iodide (DiOC2), quinolinium 6-(dimethylamino)-2-[4-[4-(dimethylamino)phenyl]-1,3-butadienyl]-1-ethyl perchlorate (LDS-751), SYTO13, rhodamine 123, calcein-AM, dihydrorhodamine 123, Mito-tracker deep red FM, ER tracker red, Rhod-2-AM, X-Rhod-1-AM, tetramethylrhodamine methyl ester perchlorate (TMRM), rhodamine B hexyl ester were purchased from Invitrogen/ThermoFisher (Carlsbad, CA). Flutax-1 was purchased from Tocris biosciences (Minneapolis, MN). Cyclosporine A was purchased from the Alexis Corporation (Lausen, Switzerland). NBD-cyclosporine A was generously provided by Drs. Anika Hartz and Björn Bauer, University of Kentucky (Lexington, KY). All remaining chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
The P-gp specific monoclonal antibody C219 was provided by Fujirebio Diagnostic Inc. (Malvern, PA), and the MRK16 antibody was purchased from Kyowa Medex Company (Tokyo, Japan). 4E3 antibody was purchased from Abcam (Cambridge, MA) and UIC2 was purchased from eBioscience (San Diego, CA) [17 (link)]. FITC-labeled anti-mouse secondary antibody IgG2aκ isotype was obtained from BD Biosciences (San Jose, CA).
The P-gp specific monoclonal antibody C219 was provided by Fujirebio Diagnostic Inc. (Malvern, PA), and the MRK16 antibody was purchased from Kyowa Medex Company (Tokyo, Japan). 4E3 antibody was purchased from Abcam (Cambridge, MA) and UIC2 was purchased from eBioscience (San Diego, CA) [17 (link)]. FITC-labeled anti-mouse secondary antibody IgG2aκ isotype was obtained from BD Biosciences (San Jose, CA).
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