Detailed description of experimental procedures is provided in Supplementary methods. All bacterial strains and plasmids used in this study are listed in Supplementary Tables 1 and 2 . All proteins were expressed, purified and characterized using SUMO-tagging strategy described earlier for E. coli RelA (33 (link)) and B. subtilis Rel (12 (link)) (Supplementary Figure S1A-G ). The monomeric nature of 50 nM B. subtilis Rel and 100 nM E. coli RelA was confirmed by mass photometry (34 (link)) using Refeyn OneMP instrument (Refeyn Ltd.) (Supplementary Figure S1H and I ).
Protocol detail
Purification and Characterization of Rel Proteins
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Walloon Excellence in Lifesciences and Biotechnology
Other organizations : Kubota (Japan), Gakushuin University
Top 5 similar protocols
Variable analysis
independent variables
- Expression, purification, and characterization of proteins using SUMO-tagging strategy
- Monomeric nature of 50 nM B. subtilis Rel and 100 nM E. coli RelA, confirmed by mass photometry
- All bacterial strains and plasmids used in this study are listed in Supplementary Tables 1 and 2
- Experimental procedures are detailed in Supplementary methods
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!