The chromatin immunoprecipitation was conducted using 2-week-old seedlings of the wild type Col-0, DTF1-MYC/clsy1234, and DTF1-MYC/Col-0 grown in 1/2 strength MS solid medium as previously described43 (link). The antibody used for DTF1 ChIP assays was anti-MYC (16–219, Millipore). The DNA was diluted in 50 μl of TE, and a 1.5 μl volume was used for each qPCR reaction. The relative enrichment to input was calculated from three biological replicates.
Nuclei were extracted from 2-week-old Arabidopsis seedlings grown in 1/2 strength MS medium as previously described38 (link) and were digested with Micrococcal Nuclease (MNase; NEB) before chromatin immunoprecipitation was conducted using antibodies against H3 (Abcam, ab1791) as previously reported44 (link).
Nuclei were extracted from 2-week-old Arabidopsis seedlings grown in 1/2 strength MS medium as previously described38 (link) and were digested with Micrococcal Nuclease (MNase; NEB) before chromatin immunoprecipitation was conducted using antibodies against H3 (Abcam, ab1791) as previously reported44 (link).
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