Transgenic Mouse Strains for Immunology

Nur77-GFP (Tg(Nr4a1-EGFP)GY139Gsat) transgenic mice, Zap-70 deficient mice lacking mature T cells (Zap70tm1Weis), and Foxp3-RFP mice (C57BL/6-Foxp3tm1Flv/J) have been previously described (Kadlecek et al., 1998 (link); Wan and Flavell, 2005 (link); Zikherman et al., 2012 (link)). C57BL/6J mice (WT mice in the text) and CD45.1 mice (B6.SJL-Ptprca Pepcb/BoyJ) were purchased from the Jackson Laboratory. A Nur77-GFP strain that is interbred with the OT-I (C57BL/6-Tg(TcraTcrb)1100Mjb/J) TCR transgenic strain was described previously (Au-Yeung et al., 2017 (link)). This OT-I-Nur77-GFP strain was interbred with a TCRα^−/− strain (B6.129S2-Tcratm1Mom/J) purchased from the Jackson Laboratory. A Nur77-GFP strain interbred with the Foxp3-RFP strain has previously been described (Zinzow-Kramer et al., 2019 (link)). All mice were housed under specific pathogen-free conditions in the Division of Animal Resources at Emory University. Sts1^−/−, and Cbl-b^−/− strains were described previously (Carpino et al., 2004 (link); Chiang et al., 2000 (link)). These strains were maintained in the Laboratory Animal Resource Center at the University of California, San Francisco. Both female and male mice were used throughout the study. All animal experiments were conducted in compliance with the Institutional Animal Care and Use Committees at Emory University and the University of California, San Francisco.