Tracking Red Blood Cell Lifespan

Hb levels were assessed by performing a blood count (ADVIA 2120, Siemens) or using a Hb analyzer (EKF Diagnostics). Blood lactate was measured with a lactate meter (Nova Biomedical). To trace RBCs and thereby assess RBC life span, EZ-Link Sulfo-NHS-Biotin (100 mg/kg body weight; Thermo Fisher Scientific) was intravenously injected 3 days before tumor cell inoculation (51 (link)). Every 3 to 7 days, 2 μl of blood were stained with Ter119-phycoerythrin (PE) (1:200; clone TER-119, BioLegend) and streptavidin-allophycocyanin (APC) (1:200; BioLegend) and analyzed on BD FACSCanto II.

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Furrer R., Jauch A.J., Nageswara Rao T., Dilbaz S., Rhein P., Steurer S.A., Recher M., Skoda R.C, & Handschin C. (2021). Remodeling of metabolism and inflammation by exercise ameliorates tumor-associated anemia. Science Advances, 7(37), eabi4852.

Publication 2021

ADVIA 2120 lactate meter EZ-Link Sulfo-NHS-Biotin streptavidin-allophycocyanin (APC)

Allophycocyanin Blood Body weight Cell Clone Diagnostics Lactate Phycoerythrin Streptavidin Sulfo nhs biotin Tumor inoculation

Corresponding Organization :

Other organizations : University of Basel, University Hospital of Basel

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Variable analysis

independent variables

Injected EZ-Link Sulfo-NHS-Biotin (100 mg/kg body weight) 3 days before tumor cell inoculation

dependent variables

Hb levels
Blood lactate
RBC life span (traced by biotinylated RBCs)

control variables

Hb levels measured by blood count (ADVIA 2120, Siemens) or Hb analyzer (EKF Diagnostics)
Blood lactate measured with a lactate meter (Nova Biomedical)
RBCs stained with Ter119-phycoerythrin (PE) and streptavidin-allophycocyanin (APC) and analyzed on BD FACSCanto II

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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