mRNA Isolation and Expression Profiling

mRNA was isolated using the mRNA Isolation Kit for Blood/Bone Marrow (Roche Applied Science), quantified using the RiboGreen RNA quantitation method (Life Technologies, Paisley, United Kingdom), and processed using NuGEN Ovation reagents, to enhance sensitivity [20 (link)]. For each sample, 500 pg of mRNA was processed and hybridized to a Human Genome U133 Plus 2.0 Array GeneChip, as described previously [9 (link)].

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Cliff J.M., Cho J.E., Lee J.S., Ronacher K., King E.C., van Helden P., Walzl G, & Dockrell H.M. (2015). Excessive Cytolytic Responses Predict Tuberculosis Relapse After Apparently Successful Treatment. The Journal of Infectious Diseases, 213(3), 485-495.

Publication 2015

mRNA Isolation Kit for Blood/Bone Marrow

Blood Bone marrow Genechip Human genome Isolation Mrna Sensitivity

Corresponding Organization : London School of Hygiene & Tropical Medicine

Other organizations : Stellenbosch University

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Variable analysis

independent variables

MRNA isolation method (mRNA Isolation Kit for Blood/Bone Marrow (Roche Applied Science))
MRNA quantification method (RiboGreen RNA quantitation method (Life Technologies, Paisley, United Kingdom))
MRNA processing method (NuGEN Ovation reagents)

dependent variables

MRNA expression levels measured using Human Genome U133 Plus 2.0 Array GeneChip

control variables

Amount of mRNA processed (500 pg)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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