Immunoblotting was performed as described29 (link). The following antibodies were used: anti-EGFR 1:200, anti-mTOR pS(2448) 1:200, anti-ERK pT(202)Y(204) (E-4) 1:200, anti-ERK2 (c-14) 1:200, GAPDH (FL-335) 1:200 (Santa Cruz); pcMet(Tyr1234/1235) 1:1000, anti- EGFR pY(1068) 1:1000,Src (L4A1) 1:1000, and Src pY(416) 1:500,anti-FAK 1:1000, anti-pFAK (Tyr397) 1:1000 (Cell Signaling).
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