Antibiotic Pharmacodynamics Comparative Analysis

Antibiotics were purchased from Sigma (gentamicin (GEN), oxacillin (OXA), vancomycin (VAN), and tetracycline (TET)), AppliChem (ciprofloxacin (CIP), rifampin (RIF)), TCI (daptomycin (DAP)), MP Biochemicals (erythromycin (ERM), and Chem-Impex International (linezolid (LIN)). The minimum inhibitory concentration (MIC) for each drug was determined by the standard two-fold dilution protocol [39 (link)]. In the experiments, each antibiotic was used at 2×MIC and 10×MIC. We chose these antibiotics to have a broad range of pharmacodynamic properties. The first eight antibiotics listed above are grouped by their pharmacodynamic characteristics: in classic assays of killing dynamics in liquid cultures at super MIC concentrations, CIP, DAP and GEN kill rapidly (fast-acting bactericidal antibiotics); OXA and VAN kill more slowly (slow-acting antibiotics); and LIN, ERM and TET prevent growth (bacteriostatic antibiotics) [40 (link)].

Free full text: Click here

Dickey J, & Perrot V. (2019). Adjunct phage treatment enhances the effectiveness of low antibiotic concentration against Staphylococcus aureus biofilms in vitro. PLoS ONE, 14(1), e0209390.

Publication 2019

gentamicin ciprofloxacin rifampin

Antibiotic Antibiotics Assays Ciprofloxacin Daptomycin Dilution Drug Erythromycin Fast Gentamicin Linezolid Minimum inhibitory concentrations Oxacillin Rifampin Tetracycline Vancomycin

Corresponding Organization : Emory University

Top 5 similar protocols

Variable analysis

independent variables

Antibiotic concentration (2×MIC and 10×MIC)

dependent variables

Minimum inhibitory concentration (MIC) for each drug

control variables

Antibiotics used: gentamicin (GEN), oxacillin (OXA), vancomycin (VAN), tetracycline (TET), ciprofloxacin (CIP), rifampin (RIF), daptomycin (DAP), erythromycin (ERM), and linezolid (LIN)
Standard two-fold dilution protocol to determine MIC
Pharmacodynamic properties of the antibiotics (fast-acting bactericidal, slow-acting, and bacteriostatic)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!