Quantification of Oxidative Modifications

N^α-(tert-butoxycarbonyl)-L-Lys (Boc-Lys), ε-aminocaproic acid (6-ACA), AA, FA, BSA, trifluoroacetic acid, 3,3′,5,5′-tetramethylbenzidine (TMB), human MPO, and H₂O₂ were purchased from Sigma-Aldrich (St. Louis, MO). Malondialdehyde bis(dimethyl) acetal, Dynabeads M-270 Amine, CarboxyLink Kit, the Imject EDC mcKLH Spin Kit, goat anti-rabbit IgG (H + L) antibody with HRP, goat anti-human IgG/IgM secondary antibody, and goat anti-mouse IgM secondary antibody were obtained from Thermo Scientific (Rockford, IL). Protein A column and autoLDL™ Cholesterol kit were purchased from GE Healthcare Life Sciences (Pittsburgh, PA) and Pointe Scientific (Lincoln Park, MI), respectively. EnVision + Single Reagents anti-mouse/rabbit-HRP were from Dako North America, Inc. (Carpentaria, CA). The anti-M2AA mouse monoclonal antibody (1F83)²⁴ and rabbit polyclonal antibody^{48 (link)} were provided by Drs. Koji Uchida (Nagoya University) and Todd A. Wyatt (University of Nebraska), respectively.

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Nakamura J., Shimomoto T., Collins L.B., Holley D.W., Zhang Z., Barbee J.M., Sharma V., Tian X., Kondo T., Uchida K., Yi X., Perkins D.O., Willis M.S., Gold A, & Bultman S.J. (2017). Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes. Scientific Reports, 7, 10787.

Publication 2017

BSA trifluoroacetic acid Malondialdehyde bis(dimethyl) acetal Dynabeads M-270 Amine CarboxyLink Kit goat anti-rabbit IgG (H + L) antibody with HRP goat anti-mouse IgM secondary antibody Protein A column

Amine Aminocaproic acid Anti antibody Anti igg Anti igm Antibody Cholesterol Dimethyl acetal Goat H2o2 Human Igm antibody Malondialdehyde Mouse Protein a Rabbit Tert Tetramethylbenzidine Trifluoroacetic acid

Corresponding Organization : University of North Carolina at Chapel Hill

Other organizations : Osaka Prefecture University, Nagoya University

Top 5 similar protocols

Variable analysis

independent variables

N^α-(tert-butoxycarbonyl)-L-Lys (Boc-Lys)
ε-aminocaproic acid (6-ACA)
Trifluoroacetic acid
3,3′,5,5′-tetramethylbenzidine (TMB)
Human MPO
H₂O₂
Malondialdehyde bis(dimethyl) acetal
Dynabeads M-270 Amine
CarboxyLink Kit
The Imject EDC mcKLH Spin Kit
Protein A column
AutoLDL™ Cholesterol kit
Anti-M2AA mouse monoclonal antibody (1F83)
Rabbit polyclonal antibody

dependent variables

Not explicitly mentioned

control variables

Kept constant to ensure valid results

positive controls

Goat anti-rabbit IgG (H + L) antibody with HRP
Goat anti-human IgG/IgM secondary antibody
Goat anti-mouse IgM secondary antibody

negative controls

EnVision + Single Reagents anti-mouse/rabbit-HRP

Annotations

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