HMVECs of cardiac origin (HMVEC-C) were obtained from Lonza (cat# CC-7030). ECs were cultured and maintained in an EBM-2 basal medium supplemented with EGM-2 MV SingleQuots™ Kit (cat# CC-3156/CC-4147). Experiments were performed at P6, considering primary ECs may become senescent at passages P8–1067 (link).
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