Analysis of Citrus Peel Extracts by UPLC-ESI-Q-TOF-MS

Ultra-high-performance liquid chromatograms (UPLCs) were obtained on an Agilent LC-MS system composed of an Agilent 1290 Infinity II UPLC coupled to an Agilent 6545 ESI-Q-TOF-MS in negative ionization mode. Aliquots (1 µL) of RO and YO peel extracts (1 mg/mL in MeOH) were analyzed on a Kinetex phenyl-hexyl (1.7 µm, 2.1 × 50 mm) column eluted with 1 min isocratic elution of 90% A (A: 100% H₂O + 0.1% formic acid) followed by 6 min linear gradient elution to 100% B (95% MeCN + 5% H₂O + 0.1% formic acid) with a flow rate of 0.4 mL/min. ESI conditions were set with the capillary temperature at 320 °C, source voltage at 3.5 kV, and a sheath gas flow rate of 11 L/ min. Ions were detected in the full scan at an intensity above 1000 counts at 6 scans/s, with an isolation width of 1.3 ~m/z, a maximum of 9 selected precursors per cycle, and using ramped collision energy (5 × m/z/100 + 10 eV) [41 (link)]. The acquired MS/MS data were processed as Agilent Mass Hunter data files (.d).

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Mounir R., Alshareef W.A., El Gebaly E.A., El-Haddad A.E., Ahmed A.M., Mohamed O.G., Enan E.T., Mosallam S., Tripathi A., Selim H.M., Bukhari S.I., Alfaraj R., Ragab G.M., El-Gazar A.A, & El-Emam S.Z. (2023). Unlocking the Power of Onion Peel Extracts: Antimicrobial and Anti-Inflammatory Effects Improve Wound Healing through Repressing Notch-1/NLRP3/Caspase-1 Signaling. Pharmaceuticals, 16(10), 1379.

Publication 2023

LC-MS system 1290 Infinity II UPLC 6545 ESI-Q-TOF-MS

Capillary Formic acid Ions Isolation Ms ms Scans Z 100

Corresponding Organization : October 6 University

Other organizations : Misr University for Science and Technology, Cairo University, University of Michigan–Ann Arbor, Mansoura University, Alfaisal University, Al-Azhar University, King Saud University

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Variable analysis

independent variables

RO peel extracts (1 mg/mL in MeOH)
YO peel extracts (1 mg/mL in MeOH)

dependent variables

Ultra-high-performance liquid chromatograms (UPLCs)

control variables

Agilent LC-MS system composed of an Agilent 1290 Infinity II UPLC coupled to an Agilent 6545 ESI-Q-TOF-MS in negative ionization mode
Kinetex phenyl-hexyl (1.7 µm, 2.1 × 50 mm) column
1 min isocratic elution of 90% A (A: 100% H2O + 0.1% formic acid) followed by 6 min linear gradient elution to 100% B (95% MeCN + 5% H2O + 0.1% formic acid) with a flow rate of 0.4 mL/min
ESI conditions with the capillary temperature at 320 °C, source voltage at 3.5 kV, and a sheath gas flow rate of 11 L/min
Ions detected in the full scan at an intensity above 1000 counts at 6 scans/s, with an isolation width of 1.3 m/z, a maximum of 9 selected precursors per cycle, and using ramped collision energy (5 × m/z/100 + 10 eV)

controls

No positive or negative controls were explicitly mentioned.

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