Quantitative Transcriptome Analysis of Plants

Total RNA from flag leaves and roots of plants 1 week after flowering was extracted using Trizol reagent (Takara), and cDNAs were synthesized using a PrimeScript First-Strand cDNA Synthesis kit (Takara) according to the manufacturer’s protocol. qPCR was performed using SYBR master mix (Tiangen) with an ABI Prism 7500 real-time PCR system (Lifetech) as previously described (Yu et al., 2017 (link)). The relative transcript levels of genes were calculated using the 2^−ΔΔCT method (Li et al., 2011 (link)). All primers used for qPCR are given in Supplementary Table S15.

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Zhou Y., Chen M., Guo J., Wang Y., Min D., Jiang Q., Ji H., Huang C., Wei W., Xu H., Chen X., Li L., Xu Z., Cheng X., Wang C., Wang C, & Ma Y. (2019). Overexpression of soybean DREB1 enhances drought stress tolerance of transgenic wheat in the field. Journal of Experimental Botany, 71(6), 1842-1857.

Publication 2019

Trizol reagent PrimeScript First-Strand cDNA Synthesis kit SYBR master mix

Cdna Genes Plants roots Primers Prism Synthesis Trizol

Corresponding Organization : Institute of Crop Sciences

Other organizations : Shijiazhuang Academy of Agriculture and Forestry Sciences, Shanxi Academy of Agricultural Sciences

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Variable analysis

independent variables

Total RNA from flag leaves and roots of plants 1 week after flowering

dependent variables

Relative transcript levels of genes

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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