Immunoblot detection of RIG-I, MDA5 and beta-actin were performed by using the method described previously [21 (link)]. Anti-RIG-I, anti-MDA5 and anti-beta actin antibodies were purchased from Cell Signaling Technology (Danvers, MA), Life Technologies (Carlsbad, CA) and Sigma (St. Louis, MO), respectively.
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Immunoblot Analysis of Innate Immune Sensors
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Anti antibodies
Beta actin
Immunoblot
Mda5
Rig i
Corresponding Organization :
Other organizations : National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, CDC Foundation
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Variable analysis
independent variables
- Anti-RIG-I antibody
- Anti-MDA5 antibody
- Anti-beta actin antibody
- Immunoblot detection of RIG-I
- Immunoblot detection of MDA5
- Immunoblot detection of beta-actin
- The method described previously [21 (link)]
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