Immunoblot Analysis of Key Proteins

Western blot was done as previously described[20 (link)]. Antibodies specific for the following targets were from Santa Cruz (Heidelberg, Germany): BAX, histone deacetylase 2 (HDAC2), HDAC1, and p53. Anti-survivin was obtained from Novus Biologicals (Cambridge, United Kingdom). HSP90 antibody was provided by Enzo Life Sciences (Lörrach, Germany).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Gock M., Mullins C.S., Bergner C., Prall F., Ramer R., Göder A., Krämer O.H., Lange F., Krause B.J., Klar E, & Linnebacher M. (2018). Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines. World Journal of Gastroenterology, 24(43), 4880-4892.

Publication 2018

BAX HDAC2 HDAC1 Anti-survivin HSP90 antibody

Antibodies Antibody Biologicals Histone deacetylase 2 Hsp90 Novus Survivin Western blot

Corresponding Organization :

Other organizations : University of Rostock, Johannes Gutenberg University Mainz, University Medical Center of the Johannes Gutenberg University Mainz

Top 5 similar protocols

Variable analysis

independent variables

Antibodies specific for the following targets: BAX, histone deacetylase 2 (HDAC2), HDAC1, p53, and survivin

dependent variables

Not explicitly mentioned

control variables

Western blot protocol was done as previously described[20]
HSP90 antibody was provided by Enzo Life Sciences (Lörrach, Germany)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!