C. jejuni strain 81-176 bacterial stocks were stored at −80 °C. After thawing, the bacteria were streaked out and incubated on selective karmali agar plates (purchased from Oxoid, Wesel, Germany) at 37 °C for 48 h in a jar under microaerophilic conditions (CampyGas Packs; Oxoid, Wesel, Germany). Grown C. jejuni bacteria were harvested in sterile PBS (Thermo Fisher Scientific, Waltham, MA, USA) immediately before infection. Age- and sex-matched human intestinal microbiota-associated (hma) IL-10−/− mice (3-month-old littermates) were then infected perorally with 109 colony forming units (CFU) of the pathogen (in 0.3 mL) on d0 and d1 (Fig. 1A ) by gavage as reported earlier [48 (link)].
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Infection of Humanized Mice with C. jejuni
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Other organizations : Charité - Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, Freie Universität Berlin
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Variable analysis
independent variables
- Infection of age- and sex-matched human intestinal microbiota-associated (hma) IL-10^{-/-} mice with 10^9 colony forming units (CFU) of Campylobacter jejuni strain 81-176 on day 0 and day 1
- Not explicitly mentioned
- Maintenance of Campylobacter jejuni strain 81-176 bacterial stocks at -80 °C
- Thawing of bacterial stocks and streaking on selective Karmali agar plates
- Incubation of bacteria on Karmali agar plates at 37 °C for 48 h under microaerophilic conditions
- Harvesting of grown Campylobacter jejuni bacteria in sterile PBS immediately before infection
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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