Tris (Dibenzylideneacetone) dipalladium (Tris DBA) was prepared by Dr Arbiser as previously described [25 (link)]. Propidium iodide (PI), RNAase and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solutions were from Sigma-Aldrich (St Louis, MO), total protein concentration was assessed by Quick Start™ Bradford dye reagent (BioRad, Hercules, CA), mAbs for Western blotting from Cell Signaling Technologies (Danvers, MA), and Annexin/PI assay from BD Biosciences (San Jose, CA). Red blood cell (RBC) lysis buffer 10 × was obtained from BioLegend (San Diego, CA). Drugs including bortezomib and carfilzomib were purchased from Selleck Chemicals (Houston, TX).
Protocol detail
Synthesis and Application of Tris DBA
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Propidium iodide
RNAase
MTT
Quick Start™ Bradford dye reagent
mAbs for Western blotting
Annexin/PI assay
bortezomib
carfilzomib
Annexin
Assay
Bortezomib
Bromide
Buffer
Carfilzomib
Drugs
Mabs
Propidium iodide
Protein
Red blood cell
Rnaase
Tris
Tris dba
Corresponding Organization : Washington University in St. Louis
Other organizations : Emory University
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Variable analysis
independent variables
- Tris (Dibenzylideneacetone) dipalladium (Tris DBA)
- Cell viability
- Apoptosis
- Propidium iodide (PI)
- RNAase
- MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solutions
- Total protein concentration
- MAbs for Western blotting
- Annexin/PI assay
- Red blood cell (RBC) lysis buffer 10 ×
- Bortezomib
- Carfilzomib
- Positive control: Not specified
- Negative control: Not specified
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