Cresyl Violet Staining of Neural Cells

Cresyl violet (CV) stains the endoplasmic reticulum and the cellular nuclei in dark purple, which includes, in the brain, cell bodies of neurons and glial cells (Eilam-Altstädter et al., 2021 ). The protocol followed guidelines of US Army Inst Pathol (Luna and Armed Forces Institute of, 1968 ), in which the sections were hydrated first, submerged for 1 h15 in 0.01% Cresyl violet (Sigma Aldrich catalog #C5042) in a 0.1 M acetate buffer (Anachemia, AC-8218) and 0.1 M acetic acid (BDH 10001CU), and then dehydrated.

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Frigon E.M., Gérin-Lajoie A., Dadar M., Boire D, & Maranzano J. (2024). Comparison of histological procedures and antigenicity of human post-mortem brains fixed with solutions used in gross anatomy laboratories. Frontiers in Neuroanatomy, 18, 1372953.

Publication 2024

Corresponding Organization : Université du Québec à Trois-Rivières

Other organizations : Douglas Mental Health University Institute, McGill University, Montreal Neurological Institute and Hospital

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Variable analysis

independent variables

Cresyl violet concentration (0.01%)

dependent variables

Staining pattern of endoplasmic reticulum and cellular nuclei

control variables

Acetate buffer concentration (0.1 M)
Acetic acid concentration (0.1 M)
Staining duration (1 h 15 min)
Tissue hydration and dehydration steps

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