For cell doubling, xCELLigence RTCA SP device (ACEA Biosciences) was measured by seeding 10,000 cells into a E-Plate 16 (16-well plate) and allow them to grow for 72 h in HCC culture medium (pHCC cell lines) or in DEMEM (Huh-7, HepG2H1.3, Hep3B). Doubling time was calculated from 5 technical replicates and by the usage of the Xcelligence RTCA Software v2.0 for real-time data analysis [21 (link)].
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