In Situ Hybridization Assay for miR-31

The miR‐31 in situ hybridisation assay was performed as described.
²³ (link) Tumours embedded in OCT (#4583; SAKURA) were cut into 10 µm slices. Digoxigenin‐labelled LNA probes mmu‐miR‐31 (#39153; Exiqon) were used following the manufacturer's protocol. Both digoxigenin‐labelled miR‐31 and scrambled probes were hybridised at 55°C overnight in a humidified chamber. The signals were detected by staining with anti‐digoxigenin‐AP antibody (#110932374910; Roche) and finally developed with BM purple substrate (#11442074001; Roche).

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Zhang P., Guan L., Sun W., Zhang Y., Du Y., Yuan S., Cao X., Yu Z., Jia Q., Zheng X., Meng Z., Li X, & Zhao L. (2024). Targeting miR‐31 represses tumourigenesis and dedifferentiation of BRAFV600E‐associated thyroid carcinoma. Clinical and Translational Medicine, 14(5), e1694.

Publication 2024

anti‐digoxigenin‐AP antibody BM purple substrate

Corresponding Organization : Tongji Hospital

Other organizations : Nankai University, Zhujiang Hospital, Southern Medical University, China Agricultural University

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Variable analysis

independent variables

Use of digoxigenin-labelled LNA probes mmu-miR-31

dependent variables

Signals detected by staining with anti-digoxigenin-AP antibody and developed with BM purple substrate

control variables

Tumours embedded in OCT (#4583; SAKURA) were cut into 10 µm slices
Digoxigenin-labelled miR-31 and scrambled probes were hybridised at 55°C overnight in a humidified chamber

controls

Scrambled probe serves as a negative control

Annotations

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