Telomerase Inhibition in Progenitor and Stem Cells

An equal number of cells from PCs and SDCs were seeded in 200 μl DMEM with 10% FBS and in 200 μl serum-free medium using 96-well culture plates and ultra-low-attachment 96-well round bottom plates (all, Corning, Costar, USA), respectively, in triplicate. EGF and bFGF were added every other day for the forming of SDCs. MST-312 (EMD, Millipore, USA) is a synthetic compound that has been shown to be a potent telomerase inhibitor (28 (link)). MST-312 powder was reconstituted to 5 mg/mL in dimethyl sulfoxide, and further diluted to the desired concentration. After growing the PCs and SDCs, MST-312 was added at concentrations of 1 and 10 μM and incubated for 24, 48, and 72 h (29 (link)).