HeLa S3 cells (ATCC)
were maintained in Dulbecco’s modified eagle medium (DMEM)
with 10% v/v bovine calf serum (HyClone) and maintained in a 37 °C,
5% CO2 environment. OPM-2 cells (a kind gift from Donald
McDonnell) were maintained in RPMI 1640 media supplemented with 12%
fetal bovine serum (HyClone), 21.8 mM glucose, 8.6 mM HEPES (pH 7.4),
and 1.0 mM sodium pyruvate. All media components were from Cellgro
unless otherwise noted. All reagents used in following assays were
from Sigma-Aldrich unless otherwise noted. HeLa S100 cytosolic lysates
were generated from cells based on the Dignam protocol as previously
described.12 (link) Isolated HeLa S100 cytosolic
lysates were quantified with a Nanodrop 2000 (Thermo Scientific),
aliquoted, and stored at −80 °C.
were maintained in Dulbecco’s modified eagle medium (DMEM)
with 10% v/v bovine calf serum (HyClone) and maintained in a 37 °C,
5% CO2 environment. OPM-2 cells (a kind gift from Donald
McDonnell) were maintained in RPMI 1640 media supplemented with 12%
fetal bovine serum (HyClone), 21.8 mM glucose, 8.6 mM HEPES (pH 7.4),
and 1.0 mM sodium pyruvate. All media components were from Cellgro
unless otherwise noted. All reagents used in following assays were
from Sigma-Aldrich unless otherwise noted. HeLa S100 cytosolic lysates
were generated from cells based on the Dignam protocol as previously
described.12 (link) Isolated HeLa S100 cytosolic
lysates were quantified with a Nanodrop 2000 (Thermo Scientific),
aliquoted, and stored at −80 °C.
