Purified, in vivo grade (reconstituted in PBS 7.0 containing no preservatives or stabilizers) rat IgG2a isotype control antibody or monoclonal anti‐Ly6G (100 μg/100 μl, 1A8, BioXcell, Lebanon, NH) was injected I.P. on days −1, 0, and +1 with respect to transurethral inoculation with UPEC‐GFP (CFT073). Ly6G is expressed on myeloid cells including neutrophils (Privratsky et al., 2018 (link); Rose et al., 2012 ; Yu et al., 2016 (link)).
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