Establishing Notch Transcriptomic Signature in Breast Cancer Cell Lines

The breast cancer cell lines (BT-20, HCC70, HCC1187, HCC1599, MDA-MB-468 and MDA-MB-436) used for establishing the Notch transcriptomic signature were purchased fresh from the American Type Culture Collection (ATCC) and maintained in RPMI 1640 medium (ATCC’s modification, cat #A1049101, Thermo Fisher Scientific) with 10% fetal bovine serum (FBS, heat inactivated, cat #A3840402, Thermo Fisher Scientific) and 1% penicillin–streptomycin (cat #15140-122, Thermo Fisher Scientific). The 19 breast cancer cell lines used to select the final signature were maintained as described [46 (link)]. Accutase (cat # A6964-100ML, Sigma-Aldrich) was used to detach the cells prior to seeding on coated plates and during maintenance. All cell lines were cultivated with 5% CO2 at 37 °C.