Western Blotting of Apoptosis and Autophagy Markers

Western blotting was performed as previously described [16 (link)]. The primary antibodies used were as follows: anti-cleaved-caspase-3, anti-Bcl-2, anti-Bax, anti-phosphorylated-AMPK, anti-AMPK, anti-phosphorylated-Akt, anti-Akt, anti-LC3B, anti-p62, anti-Beclin1, anti-ATG5, anti-ATG7, anti-Acetylation, anti-Lamin B and anti-SIRT1 antibody from Cell Signaling Technology, Inc. (Danvers, MA). The anti-β-tubulin1 antibody was from Boster Biological Technology (Wuhan, China). The secondary antibodies were provided by Boster Biological Technology.

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Xu T., Song Q., Zhou L., Yang W., Wu X., Qian Q., Chai H., Han Q., Pan H., Dou X, & Li S. (2021). Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes. Nutrition & Metabolism, 18, 13.

Publication 2021

anti-cleaved-caspase-3 anti-Bcl-2 anti-Bax anti-phosphorylated-AMPK anti-AMPK anti-phosphorylated-Akt anti-Akt anti-LC3B anti-p62 anti-Beclin1 anti-ATG5 anti-ATG7 anti-Acetylation anti-Lamin B anti-SIRT1

Acetylation Anti antibody Antibodies Bcl 2 Beclin1 Biological Caspase 3 Lamin b Sirt1

Corresponding Organization :

Other organizations : Zhejiang Chinese Medical University, Shanghai University of Medicine and Health Sciences

Top 5 similar protocols

Variable analysis

independent variables

Western blotting protocol

dependent variables

Levels of cleaved-caspase-3
Levels of Bcl-2
Levels of Bax
Levels of phosphorylated-AMPK
Levels of AMPK
Levels of phosphorylated-Akt
Levels of Akt
Levels of LC3B
Levels of p62
Levels of Beclin1
Levels of ATG5
Levels of ATG7
Levels of Acetylation
Levels of Lamin B
Levels of SIRT1

control variables

Anti-β-tubulin1 antibody

positive controls

None specified

negative controls

None specified

Annotations

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