Virulent Mtb H37Rv strain was cultured and stocks were prepared and stored at −80°C, as described previously (38 (link), 47 (link)). Mice were infected via aerosol by nebulizing with 6 ml of a suspension that contained 2.4 × 107 live bacteria in an inhalation exposure system (model A4224, Glas-Col) for 40 min. The mice were each infected with approximately 50–70 Mtb colony-forming units (CFU).
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