For in vivo PET imaging, 150 μL (∼400 μCi or 14.8 MBq) of 89Zr-MSN (or 89Zr-dSiO2) in water was i.v. injected into healthy BALB/c mice (n = 3). PET scans at various time points p.i., from 0.5 h to 21 days, were performed by using a microPET/microCT Inveon rodent model scanner (Siemens Medical Solutions USA, Inc.). The images were reconstructed using a maximum a posteriori algorithm, with no scatter correction. ROI analysis of each PET scan was performed using vendor software (Inveon Research Workplace [IRW]) on decay-corrected whole-body images, as we described previously,40 (link) to calculate the percentage injected dose per gram of tissue (%ID/g) values in mouse liver and bone.
Protocol detail
In Vivo PET Imaging of Zirconium-89 Nanoparticles
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of Wisconsin Carbone Cancer Center
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Injection of 150 μL (∼400 μCi or 14.8 MBq) of ^89Zr-MSN (or ^89Zr-dSiO2) in water into healthy BALB/c mice
- Percentage injected dose per gram of tissue (%ID/g) values in mouse liver and bone
- Healthy BALB/c mice (n = 3)
- No explicit mention of positive or negative controls
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!