Muscle lysate proteins (60 μg total protein) were separated on 4–20% linear gradient SDS polyacrylamide gels and processed for mass spectrometry as described (26 (link),27 (link)) and as outlined in supplemental Fig. 1 in the online appendix. A complete description of this analysis is provided in the online appendix.
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Mass Spectrometry Protein Separation
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Corresponding Organization : Arizona State University
Other organizations : Odense University Hospital
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- Separation of muscle lysate proteins (60 μg total protein) on 4–20% linear gradient SDS polyacrylamide gels
- Proteins measured using mass spectrometry
- The analysis was carried out as described in previous studies (26 and 27), and a complete description is provided in the online appendix.
- No specific positive or negative controls were mentioned in the provided information.
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