The total RNA was extracted from the worms using TRIzol reagent (Invitrogen, Waltham, MA, USA), and the RNA was isolated using a phase-lock gel (MaXtract High Density; Qiagen, Germantown, MD, USA) as previously described [18 (link)]. cdc-42 was used as an endogenous control for normalization, and the relative expression of each gene was calculated compared with that of the control. All experiments were independently performed in triplicate. The primers used were as presented in the Supplementary Table S1 .
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