Flies were maintained in standard cornmeal media at 25°C under light–dark cycles of 12:12 hours. To establish the transgenic fly line carrying the human 2N4R tau with or without mutations or deletions, cDNA was subcloned into pUASTattB, and injected to the oocytes carrying into P{CaryP}attP2. UAS-SOD1 (31 (link)) was obtained from Bloomington stock center (Stock #33605). For RU486 feeding, RU486 was mixed into the cornmeal media at the final concentration of 500 μm . Genotypes of the flies used in the experiments are described in Supplementary Material Table S1 .
Protocol detail
Transgenic Fly Line Generation
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Corresponding Organization : Tokyo Metropolitan University
Other organizations : Doshisha University, Gakushuin University
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Variable analysis
independent variables
- Presence or absence of mutations or deletions in the human 2N4R tau
- Not explicitly mentioned
- Flies were maintained in standard cornmeal media at 25°C under light–dark cycles of 12:12 hours
- UAS-SOD1 (31) was obtained from Bloomington stock center (Stock #33605)
- RU486 was mixed into the cornmeal media at the final concentration of 500 μM
- Positive control: UAS-SOD1 (31) from Bloomington stock center
- Negative control: Not explicitly mentioned
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