Mice were briefly anesthetized under isoflurane anesthesia. To be consistent with the FLIT procedure on the right side, masseter muscles on the right side were injected with botulinum toxin A (Merz Pharmaceutical) at 0.4U in 50 μL. H&E staining atrophy was diagnosed by a pathologist who was blinded to group assignments, using muscle fiber diameter and nucleus position as the diagnostic criteria.
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