Chitosan-Coated Microparticle Diets

MBD was prepared as a precursor of microcoated diets (Table 1). Chitosan was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China), and the deacetylation degree was more than 90%. Chitosan coating solution were prepared in 2% glacial acetic acid at first. Then, the wall material was sprayed on the surface of the core, and the feed product was obtained after drying at 50 degrees. The process index of the coating machine was 12 m³/h air volume, 100 rpm rotary speed, 54.0°C air inlet temperature, and 0.20 sec pulse blowing time with 3.0 sec interval time. The fish were fed with MCD which processed with Chitosan coating solution (0.30% Chitosan, 0.60% Chitosan, and 0.90% Chitosan) [17 (link), 18 (link)]. All preparation was done at the Nankou Base of Chinese Academy of Agricultural Science (Beijing, China).