The rat RMVs and BMVs were isolated as described previously.11 (link) Briefly,
brain and retinal crysections were individually homogenized using a motor-driven
homogenizer (Homgen plus, Schuett Biotec, Goettingen, Germany). The brain
homogenate was centrifuged at 438g for 10min, followed by centrifugation at
4400g for 15min, after which the pellet was resuspended into 7 mL PBS/1% dextran
(Dextran 70,000, Roth). Thereafter, the brain and retinal suspension were
individually transferred onto a density gradient column and centrifuged for 15
min (1300 g). Finally, the microvessels were captured after filtration over a
60 µm nylon mesh. All the procedures were performed at 0°C.
brain and retinal crysections were individually homogenized using a motor-driven
homogenizer (Homgen plus, Schuett Biotec, Goettingen, Germany). The brain
homogenate was centrifuged at 438g for 10min, followed by centrifugation at
4400g for 15min, after which the pellet was resuspended into 7 mL PBS/1% dextran
(Dextran 70,000, Roth). Thereafter, the brain and retinal suspension were
individually transferred onto a density gradient column and centrifuged for 15
min (1300 g). Finally, the microvessels were captured after filtration over a
60 µm nylon mesh. All the procedures were performed at 0°C.
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