Protocol detail

Immunofluorescence Microscopy of RAD51 and RPA2

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Immunofluorescence was performed as previously described (5 (link)). Antibodies used were as follows: RAD51 (398587, 1:800, Santa Cruz Biotechnology) and RPA2 (NA19L, 1:500, Millipore, RRID: AB_565123). Cells were imaged using a Zeiss LSM510 confocal microscope or Keyence Fluorescence Microscope BZ-X710.

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Nichols B.A., Oswald N.W., McMillan E.A., McGlynn K., Yan J., Kim M.S., Saha J., Mallipeddi P.L., LaDuke S.A., Villalobos P.A., Rodriguez-Canales J., Wistuba I.I., Posner B.A., Davis A.J., Minna J.D., MacMillan J.B, & Whitehurst A.W. (2018). HORMAD1 Is a Negative Prognostic Indicator in Lung Adenocarcinoma and Specifies Resistance to Oxidative and Genotoxic Stress. Cancer research, 78(21), 6196-6208.

Publication 2018

RAD51 NA19L Fluorescence Microscope BZ-X710

Antibodies Cells Confocal microscope Fluorescence microscope Immunofluorescence Rpa2

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Variable analysis

independent variables

Antibodies used: RAD51 (398587, 1:800, Santa Cruz Biotechnology) and RPA2 (NA19L, 1:500, Millipore, RRID: AB_565123)

dependent variables

Immunofluorescence imaging using a Zeiss LSM510 confocal microscope or Keyence Fluorescence Microscope BZ-X710

control variables

Cells were imaged using a Zeiss LSM510 confocal microscope or Keyence Fluorescence Microscope BZ-X710

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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