Total RNA Extraction and Gene Expression Analysis

Total RNA was extracted from each sample using a TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, Dalian, China). RNA concentration and purity were detected using a NanoDrop ND 2000 (NanoDrop Technologies) spectrophotometer. cDNA was synthesised in an ice box using a Reverse Transcriptase Kit (PrimeScript™ RT, Takara, Dalian, China) according to the manufacturer’s instructions. All primers were designed using Primer Premier 5.0. Relative gene expression levels were calculated from the melting curve fluorescence signals using the 2^−ΔΔCt method, with AP4 as the reference gene [57 (link)].

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Guo A., Yang Y., Wu J., Qin N., Hou F., Gao Y., Li K., Xing G, & Li S. (2023). Lipidomic and transcriptomic profiles of glycerophospholipid metabolism during Hemerocallis citrina Baroni flowering. BMC Plant Biology, 23, 50.

Publication 2023

TaKaRa MiniBEST Plant RNA Extraction Kit NanoDrop ND 2000 PrimeScript™ RT,

Cdna Fluorescence Gene Gene expression Plant rna Primer Reverse transcriptase

Corresponding Organization :

Other organizations : Luliang University, Shanxi Agricultural University

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Variable analysis

independent variables

RNA extraction method (TaKaRa MiniBEST Plant RNA Extraction Kit)
CDNA synthesis method (Reverse Transcriptase Kit)

dependent variables

Relative gene expression levels

control variables

Reference gene (AP4)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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