Immunofluorescence Staining of Mouse CD31

IF methods were described previously (8 (link)). Antibodies used were: 1:100 rat anti-mouse CD31 antibody (BD, 550274), 1:200 Alexa Fluor^® 488 goat anti-rat antibody (Invitrogen, A11006), and 1:500 monoclonal mouse anti-α-smooth muscle actin (SMA) Cy3 antibody (Sigma, C6198). Slides were mounted with Vectashield Hardset Mounting Medium with DAPI (Vector Labs) and imaged on a Zeiss CLSM 710 or 700 Spectral Confocal Laser Scanning Microscope.

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Xiao L., Kim D.J., Davis C.L., McCann J.V., Dunleavey J.M., Vanderlinden A., Xu N., Pattenden S.G., Frye S.V., Xu X., Onaitis M., Monaghan-Benson E., Burridge K, & Dudley A.C. (2015). Tumor endothelial cells with distinct patterns of TGFβ-driven endothelial-to-mesenchymal transition. Cancer research, 75(7), 1244-1254.

Publication 2015

rat anti-mouse CD31 antibody Alexa Fluor® 488 goat anti-rat antibody Vectashield Hardset Mounting Medium with DAPI CLSM 710

Alexa fluor 488 Anti antibody Antibodies Antibody Confocal laser scanning microscope Dapi Goat Mouse Mouse α smooth muscle actin Vector

Corresponding Organization : University of North Carolina at Chapel Hill

Other organizations : Duke University

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Variable analysis

independent variables

Antibodies used: 1:100 rat anti-mouse CD31 antibody, 1:200 Alexa Fluor® 488 goat anti-rat antibody, and 1:500 monoclonal mouse anti-α-smooth muscle actin (SMA) Cy3 antibody

dependent variables

Measured outcomes not explicitly mentioned

control variables

Slides were mounted with Vectashield Hardset Mounting Medium with DAPI (Vector Labs)

controls

Positive control: Not specified
Negative control: Not specified

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