Aerobic and Anaerobic Bacterial Identification

One of the sampled swabs was used for aerobic and anaerobic bacterial culture. The samples for aerobic culture were grown on Columbia agar, Gassner and Columbia/Nalidixic acid agar media (Thermo Fisher Scientific, Brussels, Belgium) at 37 ± 2 °C. Samples for anaerobic culture were grown under anaerobic conditions on Schaedler medium (Thermo Fisher Scientific, Brussels, Belgium) at 37 ± 2 °C. Two readings of each medium were performed at 18 to 24 h and 36 to 48 h of incubation. Bacterial identification was performed by the Maldi Biotyper^® (Bruker Daltonics, Bremen, Germany). The culture was considered “negative” if no bacterial growth was observed, “positive” when one to four bacteria species were found and “positive contaminated” when more than four species were cultured [7 (link)].