In specific experiments, WT BL/6 mice were challenged with A. fumigatus and 48 h thereafter, lungs collected and enzymatically digested. Cells were enumerated and plated at 1 × 106 cells in a volume of 0.2 ml in the presence of vehicle, an inhibitor of microsomal prostaglandin E synthase-1 (mPGES-1; Cayman Chemical) or misoprostol, a global EP receptor (PGE2 receptor) agonist (Sigma, St. Louis, MO). Supernatants were collected after 24 h, clarified by centrifugation and inflammatory cytokine and IL-22 levels were quantified by Bio-Plex and ELISA (R&D Systems), respectively (10 (link)).