Lentiviral Expression of Regulatory Factors

Self-inactivating lentiviruses expressing green fluorescent protein (GFP), hemagglutinin (HA)-tagged NFI dominant repressor (NFI/EnR), or Drosophila engrailed repressor domain alone (EnR), constitutively active FLAG-tagged NFATc4 (NFATc4-Ala), or constitutively active calcineurin (CaN) were described previously (Wang et al., 2004 (link); Ding et al., 2013 (link), 2016 (link)). These plasmids are available upon request. MEK5-DN lentivirus was also described previously (Doebele et al., 2009 (link)). Human embryonic kidney 293T cells (American Type Culture Collection) were grown in DMEM (Invitrogen) containing 10% heat-inactivated fetal bovine serum (Invitrogen).

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Ding B., Dobner P.R., Mullikin-Kilpatrick D., Wang W., Zhu H., Chow C.W., Cave J.W., Gronostajski R.M, & Kilpatrick D.L. (2018). BDNF activates an NFI-dependent neurodevelopmental timing program by sequestering NFATc4. Molecular Biology of the Cell, 29(8), 975-987.

Publication 2018

Human embryonic kidney 293T cells DMEM fetal bovine serum

293t cells Calcineurin Drosophila Embryonic Fetal bovine serum Green fluorescent protein Hemagglutinin Human Kidney Lentivirus Plasmids

Corresponding Organization :

Other organizations : University of Massachusetts Chan Medical School, Albert Einstein College of Medicine, Burke Medical Research Institute, Cornell University, MIND Research Institute, University at Buffalo, State University of New York

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Variable analysis

independent variables

Self-inactivating lentiviruses expressing green fluorescent protein (GFP)
Self-inactivating lentiviruses expressing hemagglutinin (HA)-tagged NFI dominant repressor (NFI/EnR)
Self-inactivating lentiviruses expressing Drosophila engrailed repressor domain alone (EnR)
Self-inactivating lentiviruses expressing constitutively active FLAG-tagged NFATc4 (NFATc4-Ala)
Self-inactivating lentiviruses expressing constitutively active calcineurin (CaN)
MEK5-DN lentivirus

dependent variables

Not explicitly mentioned

control variables

Human embryonic kidney 293T cells (American Type Culture Collection) were grown in DMEM (Invitrogen) containing 10% heat-inactivated fetal bovine serum (Invitrogen)

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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