Flies were rendered germ-free (GF) following the protocol described in49 (link). GF flies were maintained on fly medium supplemented with antibiotics: kanamycin 50μg/mL (Sigma #K1377), ampicillin50μg/mL (Sigma #A0166), tetracyclin 10μg/mL (Sigma #T7660), erythromycin 5μg/mL (Sigma #E5389). Axenicity was confirmed by crushing the flies and plating the lysate on LB Agar (Carl Roth) and MRS Agar (Carl Roth) plates.
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Germ-Free Fly Maintenance Protocol
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Corresponding Organization :
Other organizations : MRC London Institute of Medical Sciences, Harvard University, Université Claude Bernard Lyon 1, École Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Institut de Génomique Fonctionnelle de Lyon, Imperial College London, Max Planck Institute of Molecular Cell Biology and Genetics, Manchester Academic Health Science Centre, University of Manchester, Wellcome Sanger Institute
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Variable analysis
independent variables
- Rendering flies germ-free (GF)
- Axenicity, confirmed by crushing the flies and plating the lysate on LB Agar and MRS Agar plates
- Fly medium supplemented with antibiotics: kanamycin 50μg/mL, ampicillin 50μg/mL, tetracyclin 10μg/mL, erythromycin 5μg/mL
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