Microarray Analysis of hESC, hiPSC, and Progenitors

Details of the microarray analysis were described before [12 (link)]. Human HT-12 Expression BeadChip arrays (Illumina, San Diego, CA) were used for microarray hybridization to examine the global gene expression of hESC, hiPSC, and starting populations (CD34⁺ progenitors and fibroblasts). The NIH Gene Expression Omnibus has issued the accession numbers GSE44425 (Figure 1, Figure 5(c)) for the deposited microarray data related to the above manuscript.

Free full text: Click here

Nagaria P.K., Robert C., Park T.S., Huo J.S., Zambidis E.T, & Rassool F.V. (2016). High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional Signature. Stem Cells International, 2016, 3826249.

Publication 2016

Human HT-12 Expression BeadChip arrays

Fibroblasts Gene expression Hesc Hipsc Human Hybridization Microarray Populations

Corresponding Organization : University of Maryland, Baltimore

Other organizations : Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University

Top 5 similar protocols

Variable analysis

independent variables

Cell type (hESC, hiPSC, CD34+ progenitors, fibroblasts)

dependent variables

Global gene expression

control variables

Microarray platform (Illumina HT-12 Expression BeadChip arrays)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!