HPV RNA Detection in Tissues

HPV testing was performed by RNA in situ hybridization via the RNAscope method (Advanced Cell Diagnostics, Hayward, CA), as previously detailed.(12 (link)) Five micron sections from formalin-fixed and paraffin-embedded tissue blocks were evaluated for the presence of HPV RNA using the RNAscope HPV-HR18 Probe (Advanced Cell Diagnostics, Hayward, CA) which recognizes 18 high-risk HPV genotypes (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82). In addition, all cases were tested with an HPV type 33-specific probe, and a subset were tested with an HPV type 16-specific probe. HPV positive controls included an HPV33-positive head and neck squamous cell carcinoma, while an HPV-negative head and neck squamous cell carcinoma served as a negative control.

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Bishop J.A., Andreasen S., Hang J.F., Bullock M.J., Chen T.Y., Franchi A., Garcia J.J., Gnepp D.R., Gomez-Fernandez C.R., Ihrler S., Kuo Y.J., Lewis JS J.r., Magliocca K.R., Pambuccian S., Sandison A., Uro-Coste E., Stelow E., Kiss K, & Westra W.H. (2017). HPV-related Multiphenotypic Sinonasal Carcinoma: An Expanded Series of 49 Cases of the Tumor Formerly Known as HPV-related Carcinoma with Adenoid Cystic Carcinoma-Like Features. The American journal of surgical pathology, 41(12), 1690-1701.

Publication 2017

RNAscope method RNAscope HPV-HR18 Probe

Cell Diagnostics Formalin Genotypes Head and neck squamous cell carcinoma Hpv 16 In situ hybridization Paraffin Tissue

Corresponding Organization : Mayo Clinic in Arizona

Other organizations : Zealand University Hospital Køge, Taipei Veterans General Hospital, National Yang Ming Chiao Tung University, Dalhousie University, University of Pisa, Mayo Clinic, Brown University, Providence College, Vanderbilt University Medical Center, Imperial College London, Université de Toulouse

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Variable analysis

independent variables

RNA in situ hybridization via the RNAscope method

dependent variables

Presence of HPV RNA

control variables

Formalin-fixed and paraffin-embedded tissue blocks

controls

Positive control: HPV33-positive head and neck squamous cell carcinoma
Negative control: HPV-negative head and neck squamous cell carcinoma

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