Grapevine Reference Genes Selection

Eleven candidate genes were selected based on previous studies in Arabidopsis[33] (link) and grapevine [2] (link), [26] (link), [28] (link), [29] (link), [33] (link), [34] (link). Nine of these genes were formerly described as reference genes for grapevine downy mildew pathosystem in later inoculation time-points (1–7 days post-inoculation): V-type proton ATPase 16 kDa proteolipid subunit (VATP16), 60 S ribosomal protein L18 (60S), Ubiquinol-cytochrome c reductase complex chaperone (UQCC), Small nuclear ribonucleoprotein SMD3 (SMD3) from Gamm et al. [28] (link); Elongation factor 1α (EF1α) from Trouvelot et al. [34] (link), Ubiquitin-conjugating enzyme (UBQ) and SAND family protein (SAND) from Reid et al. [26] (link), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Selim et al. [29] (link) and Actin (ACT) from Figueiredo et al. [2] (link). The other two gene homologous to Arabidopis polypyrimidine tract-binding protein 1 (AT3g01150) and D1 subunit of photosystem I and II reaction centers (ATCg00340) [33] (link), were retrieved from the grapevine TIGR database v. 8 as PTB2 protein (TC109121) and PsaB (TC134081), respectively. Grapevine specific primers were designed with Primer Express software version 3.0 (Applied Biosystems, Sourceforge, USA) using the following parameters: amplicon length between 75 and 250 bp; size: 20±2 bp; melting temperature (Tm): 60±2 °C; GC content: ±50%.

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Monteiro F., Sebastiana M., Pais M.S, & Figueiredo A. (2013). Reference Gene Selection and Validation for the Early Responses to Downy Mildew Infection in Susceptible and Resistant Vitis vinifera Cultivars. PLoS ONE, 8(9), e72998.

Publication 2013

Actin Arabidopsis Chaperone Elongation factor 1α Gene Glyceraldehyde 3 phosphate dehydrogenase Inoculation Later Photosystem i Polypyrimidine tract binding protein Primer Protein Proteolipid Proton Ribosomal protein l18 Small nuclear ribonucleoprotein Subunit Ubiquinol cytochrome c reductase Ubiquitin conjugating enzyme V type atpase

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Other organizations : University of Lisbon

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Eleven candidate genes selected based on previous studies in Arabidopsis and grapevine

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