In order to amplify the whole genome of the detected CIAV strains, DNAs were subjected to three overlapping PCRs following the protocol reported by Li et al. [27 (link)]. The obtained amplicons were purified using ExoSAP-IT™ Express PCR Product Cleanup (Thermo Fisher Scientific, Massachusetts, MA, USA) following the manufacturer’s instructions, and sequenced in both directions by Macrogen Europe (Amsterdam, The Netherlands). When full amplification of the CIAV genome was not successful, strains were sequenced in their partial VP1 gene previously amplified.
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