Cytotoxicity Assay for Breast Cancer Cells

BT-20, MDA-MB-468 breast cancer cells, and BJ-5ta were purchased from ATCC and cultured in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin. For the cytotoxicity assay, cells were seeded at low density in 96-well plates in serine containing media following a previously reported procedure [25 (link)]. Media was then aspirated, and cells were incubated in fresh serine-replete or -deplete media containing compounds at indicated concentrations or vehicle (DMSO). Cells were grown for 5 days at 37 °C with drug and media changed daily before assaying cell viability using a Presto Blue assay (Life Technologies) according to the manufacturer’s instructions.