Immunofluorescence was performed as described in a previous study.18 (link) Briefly, slides with the samples were incubated with CD36 antibody (NB400-144, rabbit, diluted 1:300, Novus Biologicals, Littleton, CO, USA) overnight at 4 °C. Immunoreactivity was visualized using anti-rabbit immunoglobulin (Ig) G secondary antibody conjugated with Alexa Fluor® 546 (1:400; Molecular Probes/Invitrogen, Eugene, OR, USA). The cells were then incubated with DAPI for nuclear staining. The slides were observed using an immunofluorescence microscope (BX50/BS-FLA; Olympus, Tokyo, Japan).
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