HiPerSolv Chromanorm HPLC gradient grade acetonitrile was purchased from VWR Chemicals (Vienna, Austria), LC-MS Chromasolv grade methanol was obtained from Honeywell (Seelze, Germany), and LC-MS grade ammonium acetate and glacial acetic acid (p.a.) were purchased from Sigma-Aldrich (Vienna, Austria). A Purelab Ultra system (ELGA LabWater, Celle, Germany) was used for further purification of reverse osmosis water.
Reference standards of mycotoxins and fungal metabolites were either isolated in-house, obtained as gifts, or purchased from the following commercial sources: Romer Labs® Inc. (Tulln, Austria), AnalytiCon Discovery (Potsdam, Germany), Bio Australis (Smithfield, Australia), Fermentek Ltd. (Jerusalem, Israel), AdipoGen Life Sciences (Liestal, Switzerland), BioViotica Naturstoffe GmbH (Dransfeld, Germany), Cfm Oskar Tropitzsch GmbH (Marktredwitz, Germany), Toronto Research Chemicals (Toronto, Canada), Santa Cruz Bioechnology Inc. (Dallas, TE, USA), Sigma-Aldrich (Vienna, Austria), Iris Biotech GmbH (Marktredwitz, Germany), Enzo Life Sciences (Lausen, Switzerland), Chiralix B.V. (Nijmegen, The Netherlands), CSIR Biosciences (Pretoria, South Africa), THP Medical Products (Vienna, Austria), AG Research (Christchurch, New Zealand), Takara Bio Europe (Saint-Germain-En-Laye, France), and LGC Promochem GmbH (Wesel, Germany). The related details are given in Electronic Supplementary Material (ESM) TableS1 .
Stock solutions of each analyte were prepared by dissolving the solid substance, preferably at 250 μg/ml in acetonitrile, but depending on the respective solubility, a few compounds were dissolved in acetonitrile/water 1:1 (v/v), methanol or water instead. Sixty-two intermediate mixes were prepared by mixing the stock solutions of 10 analytes each for easier handling. The final multi-analyte standard was freshly prepared prior to spiking experiments by mixing of the intermediate mixes. All solutions were stored at − 20 °C.
Reference standards of mycotoxins and fungal metabolites were either isolated in-house, obtained as gifts, or purchased from the following commercial sources: Romer Labs® Inc. (Tulln, Austria), AnalytiCon Discovery (Potsdam, Germany), Bio Australis (Smithfield, Australia), Fermentek Ltd. (Jerusalem, Israel), AdipoGen Life Sciences (Liestal, Switzerland), BioViotica Naturstoffe GmbH (Dransfeld, Germany), Cfm Oskar Tropitzsch GmbH (Marktredwitz, Germany), Toronto Research Chemicals (Toronto, Canada), Santa Cruz Bioechnology Inc. (Dallas, TE, USA), Sigma-Aldrich (Vienna, Austria), Iris Biotech GmbH (Marktredwitz, Germany), Enzo Life Sciences (Lausen, Switzerland), Chiralix B.V. (Nijmegen, The Netherlands), CSIR Biosciences (Pretoria, South Africa), THP Medical Products (Vienna, Austria), AG Research (Christchurch, New Zealand), Takara Bio Europe (Saint-Germain-En-Laye, France), and LGC Promochem GmbH (Wesel, Germany). The related details are given in Electronic Supplementary Material (ESM) Table
Stock solutions of each analyte were prepared by dissolving the solid substance, preferably at 250 μg/ml in acetonitrile, but depending on the respective solubility, a few compounds were dissolved in acetonitrile/water 1:1 (v/v), methanol or water instead. Sixty-two intermediate mixes were prepared by mixing the stock solutions of 10 analytes each for easier handling. The final multi-analyte standard was freshly prepared prior to spiking experiments by mixing of the intermediate mixes. All solutions were stored at − 20 °C.
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