Biofilm Ammonia Production Quantification

ADS activity (as µmol of ammonia) was measured by quantification of ammonia generated from the incubation of biofilm with Nessler’s reagent at 37 °C as per a previous study^{44 (link)}. The ammonia produced was detected by Nessler’s reagent (Sigma-Aldrich, St. Louis, USA) using 8-point reference of ammonium sulfate (10 mM high-point reference) subjected to a standard curve (R² = 0.999). Each sample was assayed in triplicate. ADS activity determined was recorded as µmol of ammonia liberated in the biofilm.

Free full text: Click here

Bijle M.N., Neelakantan P., Ekambaram M., Lo E.C, & Yiu C.K. (2020). Effect of a novel synbiotic on Streptococcus mutans. Scientific Reports, 10, 7951.

Publication 2020

Nessler’s reagent

Ammonia Ammonium sulfate Biofilm

Corresponding Organization : University of Hong Kong

Other organizations : University of Otago

Top 5 similar protocols

Variable analysis

independent variables

Incubation of biofilm with Nessler's reagent at 37 °C

dependent variables

ADS activity (as µmol of ammonia)

control variables

Previous study⁴⁴ as per the reference provided

controls

Positive control: 8-point reference of ammonium sulfate (10 mM high-point reference) subjected to a standard curve (R² = 0.999)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!