To investigate the effects of miRNA-378 following intestinal I/R injury, the chemically modified agomir and antagomir were used to increase or decrease miRNA expression in vivo.38 (link) The miRNA agomir is a chemically modified, cholesterylated, stable miRNA mimic, and its in vivo delivery resulted in target silencing similar to the effects induced by the overexpression of endogenous miRNA. The miRNA antagomir is a chemically modified, cholesterol-conjugated, single-stranded RNA analog that complements the miRNAs and could efficiently and specifically silence the endogenous miRNA. AgomiR-378 and antAgomiR-378 were synthesized by RiboBio (Guangzhou, China). Scrambled mimics that did not target any miRNA were injected as a NC. Their sequences are listed as follows: AgomiR-378: 5′-ACUGGACUUGGAGUCAGAAGG-3′, 3′-UGACCUGAACCUCAGUCUUC-5′ AgomiR-378 NC: 5′-UCACAACCUCCUAGAAAGAGUAGA-3′, 3′-AGUGUUGGAGGAUCUUUCUCAUCU-5′, antAgomiR-378: 5′-CCUUCUGACUCCAAGUCCAGU-3′ and antAgomiR-378 NC: 5′-CAGUACUUUUGUGUAGUACAAA-3′.
The mice received either agomir, antagomir or their NCs (100 μl) via tail vein injection (40 mg/kg body weight, n=8 per group) for three consecutive days. Expression of miR-378 was detected on the fourth day by RT-qPCR.
The mice received either agomir, antagomir or their NCs (100 μl) via tail vein injection (40 mg/kg body weight, n=8 per group) for three consecutive days. Expression of miR-378 was detected on the fourth day by RT-qPCR.
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